Tongkat ali scientific research

The scientific name of tongkat ali is Eurycoma longifolia.

Tongkat ali has been studied for a wide range of applications. Below are references to some of these studies.


Effect of Tongkat Ali on stress hormones and psychological mood state in moderately stressed subjects


Eurycoma longifolia Jack in managing idiopathic male infertility

Breast cancer< >

Cytotoxic effects of the root extracts of Eurycoma longifolia Jack

Colon cancer

Anti-angiogenic quassinoid-rich fraction from Eurycoma longifolia modulates endothelial cell function


Screening for antihyperglycaemic activity in several local herbs of Malaysia


Antibacterial activity of Eurycoma longifolia Jack. A Malaysian medicinal plant.


Ethnobotanical study on some Malaysian anti-malarial plants: A community based survey

Naturally occurring Ngn2 promoter activators from Butea superba

Neurogenin2 (Ngn2), an activator-type bHLH transcriptional factor, promotes differentiation of neural stem cells into neurons by transcription of pro-neural genes. To find neural stem cell accelerators from the extract library of natural resources, we used a two-step screening including a Ngn2 promoter reporter gene screening and differentiation assay screening of neural stem cells. A reporter gene assay that can detect Ngn2 promoter activity by luciferase expression was constructed using C3H10T1/2 cells. Using this primary cell-based screening, Butea superba was found to include Ngn2 promoter activators from our tropical plant extract libraries. Bioassay-guided fractionation of this plant extract led to the isolation of 18 natural products, including pterocarpans and isoflavonoids. Dehydromaackiain (1), formononetin (6), (−)-variabilin (13), (−)-medicarpin (14), rothindin (17) and ononin (18) showed 1.8–2.8 times higher Ngn2 promoter activity at 5 μM compared with control. Of active natural compounds, 3′-methoxydaidzein (3) showed promotion of neurite outgrowth of C17.2 in a secondary screen. 3′-Methoxydaidzein (3) increased mRNA expression of pro-neural transcriptional factors (Ngn2, Ngn1, NeuroD2), a mature neuron-specific enzyme GAD1 and a pro-neural neurotrophic growth factor neurotrophin 3 (NT3) in C17.2 neural stem cells.